MILLIPORE 0.5ML新型超滤离心管 UFC500308 上海万资实业有限公司

With their vertical membrane design, Amicon Ultra 0.5 mL filters deliver great performance and lower spin times

Applications

Material Supplied

The Amicon Ultra-0.5 device is supplied with two microcentrifuge tubes. During operation, one tube is used to collect filtrate; the other tube is to recover the concentrated sample.

Performance – Protein Concentration

Spin conditions: 40º fixed angle rotor, 14,000 x g, room temperature, 500 µL starting volume. Protein marker used: Cytochrome c, n=6.

Typical Concentrate Volume/Concentration Factor vs. Spin Time

Concentrate Volume / Concentration Factor
	3K device		10K device		30K device		50K device		100K device
Spin time (min)	Conc. Volume (µL)	Conc. Factor (x)	Conc. Volume (µL)	Conc. Factor (x)	Conc. Volume (µL)	Conc. Factor (x)	Conc. Volume (µL)	Conc. Factor (x)	Conc. Volume (µL)	Conc. Factor (x)
5	215	2	66	7	42	12	28	18	58	9
10	114	4	35	14	23	22	20	25	19	26
15	80	6	22	22	19	27	17	30	15	33
20	62	8	20	24	17	30	15	33	13	36
30	48	10	15	31	15	32	15	36	11	41
Spin Conditions: 40º fixed angle rotor, 14,000 x g, room temperature, 500 µL starting volume. Protein markers used: Cytochrome c for 3K and 10K, BSA for 30K and 50K, and IgG for 100K, n=12. Shaded volumes were used for the calculation of protein recovery in the Typical Concentrate Recovery table.

Typical Retention of Protein Markers

Marker/Concentration	Molecular Weight	Device NMWL	% Retention	Spin Time (min)
α-Chymotrypsinogen (1 mg/mL)	25,000	3K	> 95	30
Cytochrome c (0.25 mg/mL)	12,400		> 95	30
Vitamin B-12 (0.2 mg/mL)	1,350		< 42	30
α-Chymotrypsinogen (1 mg/mL)	25,000	10K	> 95	15
Cytochrome c (0.25 mg/mL)	12,400		> 95	15
Vitamin B-12 (0.2 mg/mL)	1,350		< 42	15
BSA (1 mg/mL)	67,000	30K	> 95	10
Ovalbumin (1 mg/mL)	45,000		> 95	10
Cytrochorme c (0.25 mg/mL)	12,400		< 35	10
BSA (1 mg/mL)	67,000	50K	> 95	10
Ovalbumin (1 mg/mL)	45,000		~ 40	10
Cytrochorme c (0.25 mg/mL)	12,400		< 20	10
Thyroglobulin (0.5 mg/mL)	677,000	100K	> 95	10
IgG (1 mg/mL)	156,000		> 95	10
Ovalbumin (1 mg/mL)	45,000		< 30	10
Spin Conditions: 40º fixed angle rotor, 14,000 x g, room temperature, 500 µL starting volume, n=12.

Typical Concentrate Recovery

Marker/Concentration	Molecular Weight	Device NMWL	Spin Time (min)	Concentrate Volume (µL)	Concentration Factor (x)	Concentrate Recovery (%)
Cytochrome c (0.25 mg/mL)	12,400	3K	30	48	10	98
Cytochrome c (0.25 mg/mL)	12,400	10K	15	22	22	93
BSA (1 mg/mL)	67,000	30K	10	23	22	97
BSA (1 mg/mL)	67,000	50K	10	20	25	92
IgG (1 mg/mL)	156,000	100K	10	19	26	92
Spin Conditions: 40º fixed angle rotor, 14,000 x g, room temperature, 500 µL starting volume, n=12. The shaded volumes were taken from the Typical Concentrate Volume/Concentration Factor vs. Spin Time table.

Desalting or Diafiltration

Desalting, buffer exchange or diafiltration are important methods for removing salts or solvents in solutions containing biomolecules. The removal of salts or the exchange of buffers can be accomplished in the Amicon Ultra-0.5 device by concentrating the sample, then reconstituting the concentrate to the original sample volume with any desired solvent. The process of "washing out" can be repeated until the concentration of the contaminating microsolute has been sufficiently reduced. See example below.

Specifications (return to top)

Volumes
Maximum initial sample volume, µL	500
Typical final concentration volume, µL	15 – 20
Recommended relative centrifugal force	14,000 x g for concentration spin 1,000 x g for reverse spin
Maximum relative centrifugal force	15,000 x g
Active membrane area, cm²	1
Hold up volume, µL	< 1
Materials
Filter device	Copolymer styrene/butadiene
Membrane	Millipore Ultracell low-binding regenerated cellulose
Collection tube	Polypropylene
The ultrafiltration membranes in Amicon Ultra-0.5 mL devices contain trace amounts of the following:	Amicon Ultra 3K devices: glycerine Amicon Ultra 10K device: triethylene glycol (TEG)
If these materials interfere with analysis, pre-rinse the device with buffer or Milli-Q water. If interference continues, rinse with 0.1 N NaOH followed by a second spin of buffer or Milli-Q water.
Dimensions
Filter device, mm (in.)	Diameter: 9.4 (0.37)
	Length: 29.6 (1.17)
Tube, mm (in.)	Diameter: 10.8
	Length: 43.0 (1.69)
Filter device and tube, mm (in.)	Length (concentration mode; device in tube): 49.9
	Length (reverse spin; device upside down in tube): 37.3 (1.47)

Chemical Compatibility of Amicon Ultra Filters

Acids	Concentration	Acids	Concentration
Acetic acid	≤ 50%*	Phosphoric acid	≤ 30%
Formic acid	≤ 5%*	Sulfamic acid	≤ 3%
Hydrochloric acid	≤ 1.0 M	Sulfuric acid	≤ 3%
Lactic acid	≤ 50%	Trichloroacetic acid (TCA)	≤ 10%*
Nitric acid	≤ 10%	Trifluoroacetic acid (TFA)	≤ 30%*
Alkalis	Concentration	Alkalis	Concentration
Ammonium hydroxide	≤ 10%	Sodium hydroxide	≤ 0.5 M
Alcohols	Concentration	Alcohols	Concentration
n-Butanol	≤ 70%	Isopropanol	≤ 70%
Ethanol	≤ 70%	Methanol	≤ 60%
Detergents	Concentration	Detergents	Concentration
Alconox® detergent	≤ 1%	Lubrol® PX detergent	≤ 0.1%
CHAPS detergent	≤ 0.1%	Nonidet™ P-40 surfactant	≤ 2%
Sodium deoxycholate	≤ 5%	Triton® X-100 surfactant	≤ 0.1%
Sodium dodecyl sulfate (SDS)	≤ 0.1%	Tween® 20 surfactant	≤ 0.1%
Terg-A-Zyme® detergent	≤ 1%
Organic solvents	Concentration	Organic solvents	Concentration
Acetone	not recommended	Ethyl acetate	not recommended
Acetonitrile	≤ 20%	Formaldehyde	≤ 5%
Benzene	not recommended	Pyridine	not recommended
Carbon tetrachloride	not recommended	Tetrahydrofuran	not recommended
Chloroform	not recommended	Toluene	not recommended
Dimethyl sulfoxide (DMSO)	≤ 5%*
Miscellaneous	Concentration	Miscellaneous	Concentration
Ammonium sulfate	Saturated	Phenol	≤ 1%
Diethyl pyrocarbonate	≤ 0.2%	Phosphate buffer (pH 8.2)	≤ 1 M
Dithiothreitol (DTT)	≤ 0.1 M	Polyethylene glycol	≤ 10%
Glycerine	≤ 70%	Sodium carbonate	≤ 20%
Guanidine HCl	≤ 6 M	Tris buffer (pH 8.2)	≤ 1 M
Imidazole	≤ 100 mM	Urea	≤ 8 M
Mercaptoethanol	≤ 0.1 M
* Contact with this chemical may cause materials to leach out of the component parts. Solvent blanks are recommended to determine whether leachables represent potential assay interferences.

比较	目录编号	说明	NMWL，kDa	数量/包装
	UFC500308	Amicon Ultra-0.5, Ultracel-3 Membrane, 3 kDa	3	8
	UFC500324	Amicon Ultra-0.5, Ultracel-3 Membrane, 3 kDa	3	24
	UFC500396	Amicon Ultra-0.5, Ultracel-3 Membrane, 3 kDa	3	96
	UFC5003BK	Amicon Ultra-0.5, Ultracel-3 Membrane, 3 kDa	3	500
	UFC501008	Amicon Ultra-0.5, Ultracel-10 Membrane, 10 kDa	10	8
	UFC501024	Amicon Ultra-0.5, Ultracel-10 Membrane, 10 kDa	10	24
	UFC501096	Amicon Ultra-0.5, Ultracel-10 Membrane, 10 kDa	10	96
	UFC5010BK	Amicon Ultra-0.5, Ultracel-10 Membrane, 10 kDa	10	500
	UFC503008	Amicon Ultra-0.5, Ultracel-30 Membrane, 30 kDa	30	8
	UFC503024	Amicon Ultra-0.5, Ultracel-30 Membrane, 30 kDa	30	24
	UFC503096	Amicon Ultra-0.5, Ultracel-30 Membrane, 30 kDa	30	96
	UFC5030BK	Amicon Ultra-0.5, Ultracel-30 Membrane, 30 kDa	30	500
	UFC505008	Amicon Ultra-0.5, Ultracel-50 Membrane, 50 kDa	50	8
	UFC505024	Amicon Ultra-0.5, Ultracel-50 Membrane, 50 kDa	50	24
	UFC505096	Amicon Ultra-0.5, Ultracel-50 Membrane, 50 kDa	50	96
	UFC5050BK	Amicon Ultra-0.5, Ultracel-50 Membrane, 50 kDa	50	500
	UFC510008	Amicon Ultra-0.5, Ultracel-100 Membrane, 100 kDa	100	8
	UFC510024	Amicon Ultra-0.5, Ultracel-100 Membrane, 100 kDa	100	24
	UFC510096	Amicon Ultra-0.5, Ultracel-100 Membrane, 100 kDa	100	96
	UFC5100BK	Amicon Ultra-0.5, Ultracel-100 Membrane, 100 kDa	100	500

MILLIPORE 0.5ML新型超滤离心管[产品打印页面]

MILLIPORE 0.5ML新型超滤离心管Amicon Ultra-0.5 mL Centrifugal Filters for Protein Purification and Concentration