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Biodyne Nylon Transfer Membranes[产品打印页面]

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  • 产品名称:Biodyne Nylon Transfer Membranes
  • 产品型号:30 cm x 3 m roll
  • 产品展商:其他品牌
  • 产品价格:0.00 元
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简单介绍

High sensitivity and low background for enhanced detection and resolution Do not crack, shrink, or tear when subjected to multiple cycles of hybridization, stripping, and reprobing. Intrinsically

产品描述

Four chemistries provide versatile adsorption properties:

Biodyne A Membrane (Amphoteric Nylon 6,6)
Membrane zeta potential can be modulated by changes in pH. Ideal for single probe or multiple rehybridizations, and applications where background is troublesome.

Biodyne B Membrane (Positively-charged Nylon 6,6)
Pore surfaces are populated by a high density of quaternary ammonium groups. Our highest sensitivity nylon membrane for nucleic acid applications.

Biodyne C Membrane (Negatively-charged Nylon 6,6)
Can be derivatized by coupling reactions through the carboxyl groups on the pore surfaces.

Biodyne Plus Membrane (Positively-charged Nylon 6,6 with an extremely high isoelectric point)
With certain nonradioactive detection systems, it is more sensitive than Biodyne A membrane while exhibiting lower background than Biodyne B membrane.

Media
Biodyne A Membrane: Amphoteric Nylon 6,6
Biodyne B and Plus Membranes: Positively-charged Nylon 6,6
Biodyne C Membrane: Negatively-charged Nylon 6,6

Pore Size
0.2, 0.45, and 1.2 µm

Typical Thickness
152 µm (6.0 mils ± 0.5 mils)

Solvent Compatibility
Resistant to common solvents such as acetone, alcohol, chlorinated aliphatic hydrocarbons, formamide, 2M NaOH, DMSO, and dimethylformamide. Not compatibile with concentrated formic acid (> 50%), HCl (> 4M), oxidizing agents, and long exposures (days to weeks) to pH < 2.

In addition to standard sizes, these membranes are available in additional sizes. Pall Life Sciences can cut these membranes to your specifications. For information on sizes and cuts, call your local Pall Life Sciences office.

Biodyne® B Membrane Withstands Multiple Cycles of Stripping and Reprobing

Lambda-HindIII fragments were separated in an agarose gel and transferred to Biodyne B membrane using the Pall Improved Alkaline Transfer. The blot was stripped completely and reprobed four times without loss of signal intensity. Bands were detected using a chemiluminescent detection system. 1A - 4A: blot after (re)probing. 1B - 4B: blot after stripping, prior to re(probing).

Fluorescent Detection of DNA Using Biodyne Plus Membrane

ng total DNA

Dilutions of HindIII-digested I-DNA (1000 - 1 ng/lane) were separated in an agarose gel and transferred to Biodyne Plus membrane. Signal was generated using a fluorescein-labeled probe, antifluorescein-alkaline phosphatase conjugate, and precipitating substrate. The image was generated by scanning the blot with a FluorImager* system. *FluorImager is a registered trademark of Molecular Dynamics.

Part Number Description Pkg
Biodyne A Membrane
60113 0.2 µm, 30 cm x 3 m roll 1/pkg
60102 0.45 µm, 82 mm discs 50/pkg
60103 0.45 µm, 85 mm discs 50/pkg
60104 0.45 µm, 132 mm discs 50/pkg
60105 0.45 µm, 137 mm discs 50/pkg
60101 0.45 µm, 7 x 8.5 cm sheets 10/pkg
60100 0.45 µm, 20 x 20 cm sheets 10/pkg
60120 0.45 µm, 20 cm x 3 m roll 1/pkg
60106 0.45 µm, 30 cm x 3 m roll 1/pkg
60108 1.2 µm, 30 cm x 3 m roll 1/pkg
Biodyne B Membrane, 0.45 µm
60202 82 mm discs 50/pkg
60203 85 mm discs 50/pkg
60204 132 mm discs 50/pkg
60205 137 mm discs 50/pkg
60201 7 x 8.5 cm sheets 10/pkg
60200 20 x 20 cm sheets 10/pkg
60209 20 cm x 1 m roll 1/pkg
60208 20 cm x 3 m roll 1/pkg
60207 30 cm x 3 m roll 1/pkg
Biodyne C Membrane, 0.45 µm
60316 82 mm discs 50/pkg
60317 85 mm discs 50/pkg
60318 132 mm discs 50/pkg
60319 137 mm discs 50/pkg
60315 7 x 8.5 cm sheets 10/pkg
60314 20 x 20 cm sheets 10/pkg
60251 29 cm x 3 m roll 1/pkg
Biodyne Plus Membrane, 0.45 µm
60402 82 mm discs 50/pkg
60403 85 mm discs 50/pkg
60404 132 mm discs 50/pkg
60405 137 mm discs 50/pkg
60401 7 x 8.5 cm sheets 10/pkg
60400 20 x 20 cm sheets 10/pkg
60406 30 cm x 3 m roll 1/pkg

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