Four chemistries provide versatile adsorption properties:
Biodyne A Membrane (Amphoteric Nylon 6,6)
Membrane zeta potential can be modulated by changes in pH. Ideal for single probe or multiple rehybridizations, and applications where background is troublesome.
Biodyne B Membrane (Positively-charged Nylon 6,6)
Pore surfaces are populated by a high density of quaternary ammonium groups. Our highest sensitivity nylon membrane for nucleic acid applications.
Biodyne C Membrane (Negatively-charged Nylon 6,6)
Can be derivatized by coupling reactions through the carboxyl groups on the pore surfaces.
Biodyne Plus Membrane (Positively-charged Nylon 6,6 with an extremely high isoelectric point)
With certain nonradioactive detection systems, it is more sensitive than Biodyne A membrane while exhibiting lower background than Biodyne B membrane.
Media
Biodyne A Membrane: Amphoteric Nylon 6,6
Biodyne B and Plus Membranes: Positively-charged Nylon 6,6
Biodyne C Membrane: Negatively-charged Nylon 6,6
Pore Size
0.2, 0.45, and 1.2 µm
Typical Thickness
152 µm (6.0 mils ± 0.5 mils)
Solvent Compatibility
Resistant to common solvents such as acetone, alcohol, chlorinated aliphatic hydrocarbons, formamide, 2M NaOH, DMSO, and dimethylformamide. Not compatibile with concentrated formic acid (> 50%), HCl (> 4M), oxidizing agents, and long exposures (days to weeks) to pH < 2.
In addition to standard sizes, these membranes are available in additional sizes. Pall Life Sciences can cut these membranes to your specifications. For information on sizes and cuts, call your local Pall Life Sciences office.
Biodyne® B Membrane Withstands Multiple Cycles of Stripping and Reprobing
Lambda-HindIII fragments were separated in an agarose gel and transferred to Biodyne B membrane using the Pall Improved Alkaline Transfer. The blot was stripped completely and reprobed four times without loss of signal intensity. Bands were detected using a chemiluminescent detection system. 1A - 4A: blot after (re)probing. 1B - 4B: blot after stripping, prior to re(probing).
Fluorescent Detection of DNA Using Biodyne Plus Membrane
ng total DNA
Dilutions of HindIII-digested I-DNA (1000 - 1 ng/lane) were separated in an agarose gel and transferred to Biodyne Plus membrane. Signal was generated using a fluorescein-labeled probe, antifluorescein-alkaline phosphatase conjugate, and precipitating substrate. The image was generated by scanning the blot with a FluorImager* system. *FluorImager is a registered trademark of Molecular Dynamics.
| Part Number |
Description |
Pkg |
| Biodyne A Membrane |
| 60113 |
0.2 µm, 30 cm x 3 m roll |
1/pkg |
| 60102 |
0.45 µm, 82 mm discs |
50/pkg |
| 60103 |
0.45 µm, 85 mm discs |
50/pkg |
| 60104 |
0.45 µm, 132 mm discs |
50/pkg |
| 60105 |
0.45 µm, 137 mm discs |
50/pkg |
| 60101 |
0.45 µm, 7 x 8.5 cm sheets |
10/pkg |
| 60100 |
0.45 µm, 20 x 20 cm sheets |
10/pkg |
| 60120 |
0.45 µm, 20 cm x 3 m roll |
1/pkg |
| 60106 |
0.45 µm, 30 cm x 3 m roll |
1/pkg |
| 60108 |
1.2 µm, 30 cm x 3 m roll |
1/pkg |
| Biodyne B Membrane, 0.45 µm |
| 60202 |
82 mm discs |
50/pkg |
| 60203 |
85 mm discs |
50/pkg |
| 60204 |
132 mm discs |
50/pkg |
| 60205 |
137 mm discs |
50/pkg |
| 60201 |
7 x 8.5 cm sheets |
10/pkg |
| 60200 |
20 x 20 cm sheets |
10/pkg |
| 60209 |
20 cm x 1 m roll |
1/pkg |
| 60208 |
20 cm x 3 m roll |
1/pkg |
| 60207 |
30 cm x 3 m roll |
1/pkg |
| Biodyne C Membrane, 0.45 µm |
| 60316 |
82 mm discs |
50/pkg |
| 60317 |
85 mm discs |
50/pkg |
| 60318 |
132 mm discs |
50/pkg |
| 60319 |
137 mm discs |
50/pkg |
| 60315 |
7 x 8.5 cm sheets |
10/pkg |
| 60314 |
20 x 20 cm sheets |
10/pkg |
| 60251 |
29 cm x 3 m roll |
1/pkg |
| Biodyne Plus Membrane, 0.45 µm |
| 60402 |
82 mm discs |
50/pkg |
| 60403 |
85 mm discs |
50/pkg |
| 60404 |
132 mm discs |
50/pkg |
| 60405 |
137 mm discs |
50/pkg |
| 60401 |
7 x 8.5 cm sheets |
10/pkg |
| 60400 |
20 x 20 cm sheets |
10/pkg |
| 60406 |
30 cm x 3 m roll |
1/pkg |
