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BioTrace™ PVDF Transfer Membrane[产品打印页面]
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产品名称:
BioTrace™ PVDF Transfer Membrane
产品型号:
30 cm x 3 m roll
产品厂商:
上海万资实业有限公司
简单介绍
Versatile membrane for nucleic acid and protein transfers
Low background with chemiluminescent detection systems.
Broad compatibility with commonly-used solvents.
Ideal for protein sequencing.
BioTrace™ PVDF Transfer Membrane的详细介绍
Media
Hydrophobic polyvinylidene fluoride
Pore Size
0.45 µm
Typical Thickness
147 µm (5.8 mils)
Tensile Strength
28 bar (2800 kPa, 410 psi)
Serial dilutions of E. coli lysates were transferred from a 10 to 20% gradient gel to BioTrace PVDF and a competitive PVDF membrane, then probed with rabbit anti-E. coli antibodies. Proteins were visualized using peroxidase-conjugated goat anti-rabbit antibodies and 4-chloro-1-naphthol substrate solution.
Dilutions of 
DNA HindIII fragments were separated electrophoretically on a 0.8% agarose gel and transferred under alkaline conditions to Pall Life Sciences BioTrace PVDF membrane, as well as a competitive PVDF membrane. The DNA was fixed by baking at 80 °C (176 °F) for 1 hour and the fragments identified with a 32P DNA probe hybridized at 65 °C (149 °F) for 16 hours.
| Part Number |
Description |
Pkg |
| BioTrace PVDF Transfer Membrane |
| 66594 |
7 x 8.5 cm sheets |
10/pkg |
| 66542 |
20 x 20 cm sheets |
10/pkg |
| 66547 |
20 cm x 1 m roll |
1/pkg |
| 66543 |
30 cm x 3 m roll |
1/pkg |